LAB 4 : SOURCES OF CONTAMINATION AND INFECTION

Procedure :

Step 1 : Sterilizer work bench with 70% ethanol.

Step 2 : Lighten the bunsen burner

All events is done near the bunsen burner.

AIR , EAR , NORMAL BREATHING , VIOLENT COUGHING

Step 1 : Take the molten agar and pour in petri dish and cool.

Step 2 :

AIR - Remove lid, leave it for 5 minutes and replace the lid.

EAR - Rub a sterile swab moistened with sterile isotonic solution into ear and use the swab to

           inoculate the labeled plate.

  

NORMAL BREATHING -  Remove the lid, hold about 15cm from mouth and breathe normally and
                                              directly onto the plate for1 minute.

VIOLENT COUGHING - Remove the lid, hold the plate about 15 cm from mouth and cough
                                           violently onto the agar.

Step 4 : Replace the lid and incubate at 37ºC for 48 hour.

HANDS :

Step 1 : Wash hand with distilled water, take 1ml of wash water to the petri dish.

Step 2 : Pour the molten agar into petri dish.

Step 3 : Replace the lids of petri dish and rotatethe dish until the wash water is thoroughly mixed

             with the molten agar.

Step 4 : After the agar has set, invert the dish and incubate at 37ºC for 48 hours.

Let share our  LAB 4 REPORT ...^^