LAB 2 : MEASUREMENT AND COUNTING OF CELLS USING MICROSCOPE

2.1 Ocular Micrometer

Procedure :

Step 1 : Microscope fitted with an ocular micrometer

Step 2 : Place  the stage micrometer on the stage

Step 3 : Calculate the measurement of the eyepiece division with 40x and 100x objective lens.

Step 4 : Replace stage micrometer with a slide containing microorganism and determine the

             dimension of cell.

 2.2 Neubauer Chamber

Procedure :

Step 1 : Using a sterile Pasteur pipette, add a drop of diluted yeast culture to the space between the

             coverslip and the counting chamber.

Allow about one minute for the cells to settle.

Step 2 : Observe the cells with 400x objective len and calculate the cell concentration.

Clean the Neubauer and coverslip with 70% ethanol.

Let share our LAB2 report...^^